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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/11/2013 |
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Data da última atualização: |
21/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CASTRO, A. P. de; SILVA, M. R. S. S. da; QUIRINO, B. F.; KRUGER, R. H. |
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Afiliação: |
Alinne Pereira de Castro, UnB; Maria Regina Silveira Sartori da Silva, UnB; BETANIA FERRAZ QUIRINO, CNPAE; Ricardo Henrique Krüger, UnB. |
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Título: |
Combining "Omics" strategies to analyze the biotechnological potential of complex microbial environments. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Current Protein and Peptide Science, v. 14, n. 6, 2013. |
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DOI: |
10.2174/13892037113149990062 |
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Idioma: |
Inglês |
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Conteúdo: |
It is well established in the scientific literature that only a small fraction of microorganisms can be cultured by conventional microbiology methods. The ever cheaper and faster DNA sequencing methods, together with advances in bioinformatics, have improved our understanding of the structure and functional behavior of microbial communities in many complex environments. However, the metagenomics approach alone cannot elucidate the functionality of all micro-organisms, because a vast number of potentially new genes have no homologs in public databases. Metatranscriptomics and metaproteomics are approaches based on different techniques and have recently emerged as promising techniques to describe microbial activities within a given environment at the molecular level. In this review, we will discuss current developments and applications of metagenomics, metatranscriptomics and metaproteomics, and their limitations in the study of microbial communities. The combined analysis of genes, mRNA and protein in complex microbial environments will be key to identify novel biological molecules for biotechnological purposes. |
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Palavras-Chave: |
Metagenome; Metaproteome; Metatranscriptome and microbial diversity. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aCASTRO, A. P. de
245 $aCombining "Omics" strategies to analyze the biotechnological potential of complex microbial environments.$h[electronic resource]
260 $c2013
520 $aIt is well established in the scientific literature that only a small fraction of microorganisms can be cultured by conventional microbiology methods. The ever cheaper and faster DNA sequencing methods, together with advances in bioinformatics, have improved our understanding of the structure and functional behavior of microbial communities in many complex environments. However, the metagenomics approach alone cannot elucidate the functionality of all micro-organisms, because a vast number of potentially new genes have no homologs in public databases. Metatranscriptomics and metaproteomics are approaches based on different techniques and have recently emerged as promising techniques to describe microbial activities within a given environment at the molecular level. In this review, we will discuss current developments and applications of metagenomics, metatranscriptomics and metaproteomics, and their limitations in the study of microbial communities. The combined analysis of genes, mRNA and protein in complex microbial environments will be key to identify novel biological molecules for biotechnological purposes.
653 $aMetagenome
653 $aMetaproteome
653 $aMetatranscriptome and microbial diversity
700 1 $aSILVA, M. R. S. S. da
700 1 $aQUIRINO, B. F.
700 1 $aKRUGER, R. H.
773 $tCurrent Protein and Peptide Science$gv. 14, n. 6, 2013.
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